The Rowdyruff Boys officially reappeared in the show, in the episode "The Boys are Back in Town", due partially to the requests oleh fans. The character HIM brought them back to life and added an anti-cootie vaccination to prevent the girls' kisses from having the prior deadly effect. The vaccination instead made the boys grow "bigger and lebih powerful." But unfortunately, the girls found this out much too late and made the boys grow many times thier sebelumnya size. The boys get their revenge on the girls, firstly oleh grossing them out in various ways. It has been berkata that girls can make boys...

In the Rowdyruff Boys' self-titled debut episode, Mojo Jojo created them during a stay in prison. Desperate to find a way to create a power to equal to the Powerpuff Girls, Mojo Jojo placed an anonymous phone call to Professor Utonium, who was all too willing to tell "the stranger on the phone from Townsville Community College" his recipe for the girls' creation.

Finding the ingredients of sugar, spice, and everything nice "too girlish" for his needs, Mojo wanted something "tougher" and "more manly". So he made a few variations on the recipe with everything he needed available in prison: snips...

The one big difference between the Powerpuff Girls and the Rowdyruff Boys are their weaknesses. Though the Powerpuff Girls have been defeated before, they don't have any glaring weaknesses except when they are divided. The Rowdyruff Boys, on the other hand, have several significant weaknesses that they all share. Due to the boys' being having higher tolerance for damage than the girls, they are almost impossible to defeat in head-on combat, this is proven in their first encounter where they were the only ones that could defeat the Powerpuff girls in a fair fight, these weaknesses must constantly...