I'll start:

Dean Winchester: [raises hand to stop Sam] No chick flick moments.

we already have a similar game check it out: link

Yea, but this is about just quotes for each episode, not best character or scene quotes.

Sam: When I told him I was afraid of the thing in my closet, he gave me a .45.
Dean: Well, what was he supposed to do?
Sam: I was nine years old!

Sam: Jerk.
Dean: Bitch.

Dean: I love the Smurfs

Sam: You smell like a toilet

Sam: what were you thinking shooting casper in the face you freak!!!

Dean Winchester: So, what are you gonna do? Are you just gonna live some normal, apple pie life? Is that it?
Sam Winchester: No, not normal. Safe.

Dean: I tell you, if you screwed up my car, I'll kill you.

Post your quotes for Round 02 - Wendigo below.

Go link to vote for round 01!

Dean: And what do you mean I didn't pack provisions? (pulls out peanut M&Ms and walks away)

Dean: Oh sweetheart, I don't do shorts.

Dean: Chow time you freaky bastard! I taste good!!

lol

Dean: This book. This is dad’s single most valuable possession. Everything he knows about every evil thing is in here. And he’s passed it on to us. I think he wants us to pick up where he left off, you know saving people, hunting things. The family business.

^Well, so much for my addition. I'll add a different one, tho.

Sam: How do you do it? (mirthless laugh) How does Dad do it?
Dean: Well, for one thing, them. I figure, our family's so screwed to hell, might as well help out some others.

I forgot how many good lines there are in this ep! Nice contest!

Dean: You wanna tell me what's goin' on in that freaky head of yours?
Sam: Dean...
Dean: No, you're not fine, you're like a powder keg, man, it's not like you. I'm supposed to be the belligerent one, remember?

Dean: You want some whtie meat, bitch???

Post your quotes for Round 03 - Dead in the Water below.

Go link to vote for round 02!

Dean: I didn't feel like talking, just like you. But see, my mom, I know she would've wanted me to be brave, and I'd think about that every day, and I'd do my best to be brave.

Lucas: zepplin rules

Sam: “Kids are the best?" You don't even like kids.
Dean: I love kids.
Sam: Name three children that you even know.
(long pause)
Sam: Forget it.
Dean: I'm thinking!

^lol, that's my second favorite.

Dean: Oh, college boy thinks he's so smart. (Sam laughs)
Sam: You know, um... what you said about mom ...you never told me that before.
Dean: It's no big deal... Oh God, we're not gonna have to hug or anything, are we?

Post your quotes for Round 04 - Phantom Traveler below.

Go link to vote for round 03!

Sam: Are you humming Metallica?
Dean: It calms me down!

whooo i've won two rounds ! *pats self on back*

Dean: Come on, that can't be normal!!
Sam: Hey, hey, it's just a little turbulence.
Dean: Sam, this plane is going to crash, so stop treating me like I'm freakin' four!
Sam: You need to calm down.
Dean: I'm sorry, I can't.
Sam: Yes, you can.
Dean: Dude, stow the touchy-feely-self-help-yoga crap. It's not helping!

Dean: Well, I kinda have this problem with... *waves hand*
Sam: What? Flying?
Dean: It's never really been an issue until now!
Sam: You're joking, right?
Dean: Do I look like I'm joking?

-Actually, I like that whole scene, but I can't remember all of it word for word. *gasp* I feel like such a disloyal fan!

Dean Winchester: Man. I look like one of the Blues Brothers!
Sam Winchester: No, you don't. You look more like a seventh grader at his first dance.
Dean Winchester: I hate this thing.

Sam: You've been in there forever.
Dean: You can't rush perfection.

Post your quotes for Round 05 - Bloody Mary below.

Go link to vote for round 04!

Dean: Dude, I earned that money.
Sam: You won it in a poker game.
Dean: Yeah.

Dean: Do I Look Like Paris Hilton?

Dean Winchester: Hey, Sam?
Sam Winchester: Yeah.
Dean Winchester: Now that this is all over I want you to tell me what that secret was.
Sam Winchester: Look, you're my brother, and I'd die for you. But there are some things I need to keep to myself.

Dean: Alright, you know what? That's it! This is about Jessica, isn't it? You think that's your dirty little secret? That you killed her somehow? [Sam is silent] Sam, this has got to stop, man. I mean the nightmares and... and calling her name out in the middle of the night. It's gonna kill you. Now listen to me, it wasn't your fault. If you want to blame something, then blame the thing that killed her. [still no response from Sam] Alright, why don't you take a swing at me? I'm the one who dragged you away from her in the first place.

Dean: No. I don't like it, it's not gonna happen, forget it.

Go link to vote for round 05!

Post your quotes for Round 06 - Skin below.

Dean: You know, I gotta say, I'm sorry I'm going to miss it.
Sam: Miss what?
Dean: How many chances am I going to have to see my own funeral?

Dean: I really wish things could be different, you know, I wish you could just be Joe College.
Sam: Nah, that's okay. You know, truth is, even at Stanford, deep down, I never really fit in.
Dean: Well, that's cuz you're a freak.
Sam: Yeah, thanks.
Dean: Well I'm a freak, too. I'm right there with you, all the way.

Dean: 'Well he's not stupid. He picked the handsome one.' =))

Dean: All right, but first I want to find that handsome devil and kick the holy crap out of him!

Dean: The thought of him driving my car! It's killing me!

Dean Winchester: I think we're getting closer to its lair.
Sam Winchester: How can you tell?
Dean Winchester: Because there's another puke-inducing pile next to your face.

Ah, you guys picked all my favorites! All right, here's a different one (just cuz I want something to contribute):
Rebecca: What do you think this is, Hooter's?
Dean: I wish.

whens the pick for 'skin' going up? =)

Go link to vote for round 06!

Post your quotes for Round 07 - Hook Man below.

Dean: I told him you were a dumbass pledge and that we were hazing you.
Sam: What about the shotgun?
Dean: I said that you were hunting ghosts, and spirits were repelled by rock salt. You know, typical Hell Week prank.
Sam: And he believed you?
Dean: Well, you look like a dumbass pledge.

Dean: Your, half-caff double vanilla latte is getting cold over here, Francis.
Sam: Bite me.